Gamal Moustafa
Alexandria University, Egypt
Title: Combined Treatment of HFD-induced Diabetes Mellitus by Metformin and Natural Products in Rats
Biography
Biography: Gamal Moustafa
Abstract
Background: Am widely distributed over the coastal Mediterranean region. It is traditionally used in the treatment of diabetes. The aim of the present study was to evaluate the antioxidant, antihyperlipidemic and antidiabetic activity of ethyl acetate extract of AM and also study the effect of combined treatment of the extract and metformin on diabetic rats. Metformin is an oral biguanide anti hyperglycemic agent that is currently the first line therapeutic intervention in management of type 2 diabetes.
Methods: Ethyl acetate extract taken from AM tubers was used for the study. Diabetic and lipid profile, kidney and liver functions, antioxidant and anti-inflammatory parameters were performed on the experimental animals. Diabetes was induced in rat by HFD feed for 10 weeks. Rats were divided into 5 groups; control, diabetic control, treated diabetic rats I (extract 10 mg/kg+ MET 100 mg/kg), treated diabetic rats II (extract 20 mg/kg+ MET 100 mg/kg), metformin treated diabetic rats (MET 100 mg/kg). Bodyweight of each rat in the different groups was recorded daily. Biochemical and antioxidant enzyme parameters were determined on day 16.
Results: AM (combined treatment I or II?)showed better glucose utilization and insulin resistance improvement. Orally treatment of different doses of the plant extract alone and/or with metformin decreased the level of serum glucose, activity of liver alpha glucosidase, activity of pancreatic alpha amylase, MDA, CRP and leptin. Treatment showed increased level of plasma insulin, Catalase, glutathione peroxidase, liver GSH, total antioxidant capacity. Diabetic rats treated with different doses of the extract and metformin combination significantly increased muscle glucose transporter 4 (GLUT4), and remarked regenerative effect on the liver, kidney and pancreas.
Conclusion: The antioxidant, antihyperlipidemic and antidiabetic effect of ethyl acetate extract of AM and with metformin combination suggests a potential therapeutic treatment to diabetic conditions.
Keywords: Antidiabetic, Antihyperlipidemic, metformin, malondialdehyde, glucose transporter 4.